Isolation and Analysis of Essential Oils Using Gas...

Isolation and Analysis of Essential Oils using Gas Chromatography Lyndon Justin T. Guzman Institute of Chemistry, University of the Philippines, Diliman, Quezon City Date Performed: February 2; February 4, 2011 Date Submitted: February 18, 2011 Abstract The purpose of this experiment is to isolate the essential oil from eucalyptus leaves as a pure compound; moreover, the components of the essential oil, camphor and limonene, will be then separated using gas chromatography technique, identify the components by their retention times, and compute for the concentration and percentage content of each component by their peak areas and peak heights. The volatile oil from eucalyptus leaves was isolated with the use of steam distillation setup,†¦show more content†¦Since the total vapor pressure is the sum of the individual vapor pressures, the total vapor pressure must become equal to atmospheric pressure at a temperature below the boiling point of either pure substance (Ault, 1983). The mixture thus distills at a temperature below the boiling point of either pure component. This can be explained using a combination of Dalton’s and Raoult’s Law: Patm = XAP °A + XBP °B where Patm is the atmospheric pressure, XA and XB are the mole fractions of compounds A and B, and P °A and P °B are the vapor pressures of pure liquids A and B. Their individual contributions are dependent on their respective mole fractions, and both liquids contribute to the vapor pressure of the system (Institute of Chemistry, UPD, 2010). In this experiment, a major constituent of volatile oils from eucalyptus leaves will be isolated as a pure compound with high purity via steam distillation. These essential oils are camphor and limonene and by using the gas chromatography technique, the different components of the eucalyptus essential oil will be separated. This experiment also aims to manipulate the gas chromatography system and change conditions in order to effectively and efficiently separate the components, and therefore achieve a good resolution. The components will be identified by determination of their retention timesShow MoreRelatedThe Isolation Of Volatile Oils From Cinnamon3203 Words   |  13 Pages Carlos Restrepo Dr. Lanni The isolation of Volatile Oils from Cinnamon November 18, 2014 Isolation of Volatile Oil from Cinnamon Purpose: The purpose of this experiment was to isolate the components of cinnamon by using multiple techniques. The techniques involved in the experiment include: distillation, extraction, filtration, refractomery, gas chromatography, mass spectrometry, and the use of ChemDraw. Introduction: Cinnamon is the second most important spice that is soldRead MoreExtracting Eugenol From Cloves?1832 Words   |  8 PagesCH331 Lab 7 Figure 1: Photo of Thin-Layer Chromatography using a beaker to hold the solvent. 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